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KMID : 0545120160260112012
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 11 p.2012 ~ p.2018
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Fructus Amomi Cardamomi Extract Inhibits Coxsackievirus-B3 Induced Myocarditis in a Murine Myocarditis Model
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Lee Yun-Gyeong
Park Jung-Ho
Jeon Eun-Seok
Kim Jin-Hee
Lim Byung-Kwan
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Abstract
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Coxsackievirus B3 (CVB3) is the main cause of acute myocarditis and dilated cardiomyopathy. Plant extract is considered as useful materials to develop new antiviral drug. We select the candidate plant extract, which showed anti-inflammatory effect previously. We examined the antiviral effects by using a HeLa cell survival assay. Among these extracts, we chose the Amomi Cardamomi (Amomi) extract, which showed strong antiviral effect and preserved cell survival in CVB3 infection. We investigated the mechanisms underlying the ability of Amomi extract to inhibit CVB3 infection and replication. HeLa cells infected by CVB3 with or without Amomi extract. Erk and Akt activity, and its correlation with virus replication was observed. Live virus titer in cell supernatants and viral positive- and negative-strand RNA amplification were measured. Amomi extract significantly increased HeLa cell survival in different concentrations (100?10㎍/㎖). Production of the viral capsid protein VP1 (76%) and viral protease 2A?induced eIF4G1 cleavage (70%) were significantly decreased in Amomi extract (100㎍/㎖) treated cells. The levels of positive- (20%) and negative-strand (80%) RNA were dramatically decreased compared to control, as revealed by reverse transcription?PCR. In addition, Amomi extract improved mice survival (51% vs 26%) and dramatically reduced heart inflammation in CVB3-induced myocarditis mouse model. These results suggested that Amomi extract was significantly inhibited Enterovirus replication and myocarditis damage. Amomi may be develop therapeutic drug for Enterovirus.
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KEYWORD
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Coxsackievirus B3, Myocarditis, Plant extract, Antiviral effect, Enterovirus
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